audouinii responsible of mycetoma of the scalp. The PCR derived sequences were submitted to the BLAST search (blastn. It can tell you if your sequences are proper or not and also is able to evaluate number of similar sequences and show you the rate of overlapping. Besides, mitochondrial genes phylogenies are not affected by multiple nucleotide. It was also able to give correct identification of an atypical strain of M. You can use analyser programm in BLAST gene bank. PCR sequencing provided an excellent tool for identifying dermatophyte strains that do not present typical morphological characteristics. Identification of dermatophyte isolates by conventional methods was confirmed by DNA sequencing of the ITS regions in 84% of cases. ITS sequences showed, in BLAST search analysis, 99-100% of similarity. For comparison, ITS-based PCR and DNA sequencing were applied for identification of the isolated dermatophytes. interdigitale (n = 10), Microsporum audouinii (n = 5), and one strain for each of the following species: T. The Illumina NovaSeq platform was used for the shotgun sequencing of the traditional herbal patent medicine samples after a meta-genomic DNA paired-end library was constructed. Mycological identification revealed Trichophyton soudanense (n = 13), T. Then, the sequencing output files generated via Sanger sequencing were aligned and assembled using Codoncode aligner v 9.0.1 (CodonCode Corp., Dedham, MA, United States). CodonCode Aligner is a commercial application. A collection of thirty-two strains of dermatophytes were isolated from patients suffering from dermatophytosis. This is a Java program that allows the reading of sequence traces from ABI or SCF files, the display of traces on white or black background and the display of Phred quality values as bars or numbers with one of three different color schemes. The objective of this study was to compare the phenotypic method with DNA sequencing of the ITS regions for identification of dermatophyte species isolated in Dakar, Senegal. On the other hand, nucleotide sequence analysis of internal transcribed spacers (ITS) of rDNA has proved to be a useful method for identification of dermatophytes. Sequences were edited and assembled using CodonCode Aligner v 3.7.1.1. We carried out a BLAST search and identified this sequence as a fragment of the. Abstract : Classically, dermatophytes are identified by phenotypic methods even if these methods, sometimes, remain difficult or uncertain. Sequence assignments were generated through a BLASTn similarity search against the. This article is an open access article distributed under the terms and.
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